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Endotoxin from Mycoplasma mycoides

Abstract

Plackett and Buttery1,2 extracted lipopolysaccharide from the V5 strain of M. mycoides by hot phenol3. They obtained a ‘galactan’ yielding 90 per cent D-galactose, about 4 per cent lipids, less than 2 per cent nitrogen and less than 0.1 per cent phosphorus. The galactan was not pyrogenic in rabbits and had the properties of a complex hapten.

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References

  1. Plackett, P., and Buttery, S. H., Nature, 182, 1236 (1958).

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  2. Plackett, P. (personal communication).

  3. Westphal, O., Lüderitz, O., and Bister, F., Z. Naturforsch., 7, b, 148 (1952).

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  4. Thomas, L., in Polysaccharides in Biology, edit. by Springer, G. F., 299 (1957).

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  5. Plackett, P., Biochim. Biophys. Acta, 35, 260 (1959).

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VILLEMOT, J., PROVOST, A. & QUEVAL, R. Endotoxin from Mycoplasma mycoides. Nature 193, 906–907 (1962). https://doi.org/10.1038/193906a0

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