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A Semi-quantitative Method of Counting Nematode-trapping Fungi in Soil

Abstract

THE usual method of observing nematode-trapping fungi in soil, as outlined by Duddington1, consists of sprinkling soil in a Petri dish, dispersing in cool but still liquid agar medium, and then examining the whole dish for the trapping structures of these fungi after several days of incubation. Because of the uneven size and distribution of soil particles, and the large area to be examined, this method is difficult and unreliable as a means of estimating numbers of trapping fungi present. However, as outlined here, an adaptation of this method can be used to obtain semi-quantitative estimates.

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References

  1. Duddington, C. L., Trans. Brit. Mycol. Soc., 38, 97 (1955).

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  2. Dougherty, E. C., and Calhoun, H. G., Proc. Helm. Soc. Wash., 15, 55 (1949).

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  3. Anderson, E. J., and Yanagihara, I., Phytopath., 45, 238 (1955).

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KLEMMER, H., NAKANO, R. A Semi-quantitative Method of Counting Nematode-trapping Fungi in Soil. Nature 203, 1085 (1964). https://doi.org/10.1038/2031085a0

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