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Mannosamine in the Cell Wall of Clostridium welchii (Type A)

Nature volume 206, pages 400–401 (1965)Cite this article

An Erratum to this article was published on 03 July 1965

Abstract

CELL-WALL preparations of Clostridium welchii, type A (NCTC 8237), have been obtained by a combination of the methods of Salton and Horne1 and of Cummins and Harris2. A carbohydrate fraction, containing galactos-amine, galactose, rhamnose and phosphorus, was removed from the wall by extraction with formamide3,4 at 150° for 20 min. The residue remaining after this treatment was digestible with egg-white lysozyme and contained the usual mucopeptide constituents5: muramic acid, glucosamine, alanine, glycine and α-η-diaminopimelic acid. Paper chromatography of both cell wall and formamide residue showed the presence of an additional Elson–Morgan positive6 spot which moved just faster than glucosamine when chromatographed in butanol/pyridine/water (6:4:3) on paper soaked in barium chloride as recommended by Heyworth, Perkins and Walker7.

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Authors and Affiliations

  1. National Institute for Medical Research, Mill Hill, London, N.W.7

    B. T. PICKERING

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  1. B. T. PICKERING
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An erratum to this article is available online at https://doi.org/10.1038/207023e0.

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PICKERING, B. Mannosamine in the Cell Wall of Clostridium welchii (Type A). Nature 206, 400–401 (1965). https://doi.org/10.1038/206400a0

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