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Genetic Recombination with Ethyl-methanesulphonate-induced Waxy Mutants in-Maize

Nature volume 207, pages 890–891 (1965)Cite this article

Abstract

INTRACISTRON mapping in maize has been demonstrated by Nelson1,2 with a technique based on differential staining reactions of starch in pollen of different genotypes. Those with the waxy (wx) locus stain reddish brown in iodine–potassium iodide solutions; whereas non-waxy (Wx) pollen stains dark blue. Therefore, in waxy plants genetic recombination or back-mutation at this locus is manifested by the appearance of dark blue staining pollen. The technique has a distinct advantage in genetic investigations with higher plants in that large populations of hundreds of thousands of genotypes (pollen grains) can be scored with ease.

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References

  1. Nelson, Oliver E., Science, 130, 794 (1959).

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  2. Nelson, Oliver E., Genetics, 47, 737 (1962).

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  3. Amano, E., and Smith, H. H., Mutation Research (in the press).

  4. Briggs, R. W., and Smith, H. H., J. Heredity (in the press).

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Authors and Affiliations

  1. Department of Biology, Brookhaven National Laboratory, Upton, New York

    R. W. BRIGGS, E. AMANO & H. H. SMITH

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  1. R. W. BRIGGS
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  2. E. AMANO
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  3. H. H. SMITH
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BRIGGS, R., AMANO, E. & SMITH, H. Genetic Recombination with Ethyl-methanesulphonate-induced Waxy Mutants in-Maize. Nature 207, 890–891 (1965). https://doi.org/10.1038/207890a0

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