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Method of Recording Simultaneously the Positions of Labelled and Unlabelled Compounds on Autoradiographs

Abstract

A COINCIDENCE technique for paper chromatography has recently been described1, in which a ‘shadowgram’ of a chromatogram is produced by placing the chromatogram over a photographic film and exposing the covered film to radiation of a wave-length which is absorbed by the ‘spots’ on the chromatogram. In this way a ‘shadowgram’ of purine or pyrimidine ‘spots’ may be produced by exposing the covered film to an ultra-violet source, or of amino-acid spots (after development with ninhydrin) by exposure to visible light.

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References

  1. Ponnamperuma, C., Kirk, P., Mariner, R., and Tyson, B., Nature, 202, 393 (1964).

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BELL, E., WORRALL, C. Method of Recording Simultaneously the Positions of Labelled and Unlabelled Compounds on Autoradiographs. Nature 210, 736 (1966). https://doi.org/10.1038/210736a0

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