Abstract
THE procedure described by Hunter and Greenwood1 with some minor modifications has been used in this laboratory for small-scale preparation of iodine-131-labelled insulin. About 1.2 mc. iodine-131 from Kjeller is used in a volume of less than 0.025 ml. for the labelling of 5 µg of purified insulin. The iodination efficiency is usually in the region of 60–80 per cent, giving a specific activity of about 150 µc. iodine-131 per µg insulin.
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References
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HØYE, Å. Purification of Insulin labelled with Iodine-131. Nature 211, 746 (1966). https://doi.org/10.1038/211746a0
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DOI: https://doi.org/10.1038/211746a0
