Electrophoretic Haemoglobin Patterns in One Random Bred and Two Inbred Strains of Laboratory Rats

Abstract

IT has previously been shown by paper electrophoresis that two distinct electrophoretic types of haemoglobin, “single-spot” and “diffuse”, occur in mice of several inbred strains and these have been proved to be under genetic control^1,2. Rosa et al.³ classified seven strains of mice into four different groups according to their haemoglobin patterns obtained by starch-gel electrophoresis. The largest number of bands observed was five³. Using starch-gel electrophoresis in a discontinuous buffer system, Morton⁴ showed that the “diffuse” type of mouse haemoglobin separates into five bands. As far as electrophoretic investigations of haemoglobin in rats are concerned, we have not been able to find other published data except those by Giri and Pillai⁵. Using agar-gel electrophoresis they found that in the rat blood haemolysates two minor components of proteins were present in addition to one major component of haemoglobin.