Abstract
WE have used ‘Sephadex G-15’ to examine the molecular weight distribution of urinary peptides obtained from normal males, aged between 22 and 24 yr, by a method designed to protect labile groups within peptides. We obtained a peptide fraction, of approximate molecular weight 600, which gave several slowly developing yellow Ehrlich positive zones after electrophoresis in pyridine acetate buffer at pH 5.5. This staining property was lost after chromatography in systems containing acetic acid, presumably because of oxidative decomposition on the paper surface in acetic acid. High voltage electrophoresis of this peptide fraction in pyridine acetate buffer at pH. 3.7 showed that at least fifteen peptides were present.
Similar content being viewed by others
Article PDF
References
Steven, F. S., and Jackson, D. S., Biochem. J., 104, 534 (1967).
Thomas, J., Elsden, D. F., and Partridge, S. M., Nature, 200, 651 (1963).
Franzblau, C., Sinex, F. M., Faris, B., and Lampidis, R., Biochem. Biophys. Res. Commun., 21, 575 (1965).
Butler, W. T., and Cunningham, L. W., J. Biol. Chem., 241, 3882 (1966).
Cunningham, L. W., Ford, J. D., and Segrest, J. P., J. Biol. Chem., 242, 2570 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
WEISS, J., STEVEN, F. Urinary Peptides derived from the Cross-linked Regions of Connective Tissue Proteins. Nature 217, 661 (1968). https://doi.org/10.1038/217661a0
Received:
Issue date:
DOI: https://doi.org/10.1038/217661a0
