Abstract
THE immunosuppressive properties of anti-lymphocyte serum have attracted a great deal of attention. Most work has been carried out with antisera against lymphocytes of laboratory animals1–3, but some clinical studies have also been made using anti-human lymphocyte serum (AHLS)4. Clinical work, however, is handicapped by the lack of method of assaying the immunosuppressive potency of AHLS in vitro. It has been shown that the stimulation of nucleic acid which is induced by phytohaemagglutinin (PHA) in human lymphocytes in vitro is broadly similar to that induced by antigens5–8. We have therefore studied the effect of anti-mouse lymphocyte serum (AMLS) on the response of mouse peripheral lymphocytes to PHA in vitro in the hope of establishing a correlation with the graft protecting potency of the serum. If a correlation could be established it seemed that analogous assays of AHLS might be used as a guide to its graft protecting potency.
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MOSEDALE, B., FELSTEAD, K. & PARKE, J. Effect of Anti-lymphocyte Serum on the Response of Human and Mouse Lymphocytes to PHA. Nature 218, 983–984 (1968). https://doi.org/10.1038/218983a0
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DOI: https://doi.org/10.1038/218983a0
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