Abstract
ALTHOUGH the presence of the enzyme neuraminidase (EC 3.2.1.18) is characteristic of myxoviruses, the synthesis of neuraminidase during myxovirus infections has so far been examined in few systems, and only using cells obtained from chicken eggs or embryos1–3. We report here the production of influenza virus neuraminidase in cell lines of mammalian origin, together with a simple method suitable for the routine detection of neuraminidase activity in cell monolayers.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to the full article PDF.
USD 39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Noll, H., Aoyagi, T., and Orlando, J., Virology, 14, 141 (1961).
Scholtissek, C., and Rott, R., Virology, 22, 169 (1964).
Lipkind, M. A., and Tsvetkova, I. V., J. Virology, 1, 327 (1967).
Webster, R. G., Laver, W. G., and Kilbourne, E. D., J. Gen. Virology, 3, 315 (1968).
Kendal, A. P., Biddle, F., and Belyavin, G., Biochim. Biophys. Acta, 165, 419 (1968).
Kendal, A. P., in Technicon Symposium on Automation in Analytical Chemistry, 2, 175 (Mediad, New York, 1968).
Klenk, H. D., and Choppin, P. W., Virology, 38, 255 (1969).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KENDAL, A., APOSTOLOV, K. Production of Neuraminidase in Cell Monolayers infected with Influenza Virus. Nature 226, 167–168 (1970). https://doi.org/10.1038/226167a0
Received:
Revised:
Issue date:
DOI: https://doi.org/10.1038/226167a0
This article is cited by
-
Studies on the role of myxovirus neuraminidase in virus-cell receptor interaction by means of direct determination of sialic acid split from cells
Archiv f�r die gesamte Virusforschung (1973)
-
The distribution of neuraminidase among the cytoplasmic membranes of HeLa cells infected with an influenza virus
Archiv f�r die gesamte Virusforschung (1971)
-
Flocculation of influenza virus by specific anti-neuraminidase antibody
Archiv f�r die gesamte Virusforschung (1970)
