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Intact 3′ end of 16S rRNA is not required for specific mRNA binding

Nature volume 262, pages 150–153 (1976)Cite this article

A Corrigendum to this article was published on 12 August 1976

Abstract

INITIATION of protein synthesis in bacteria requires that ribosomes bind to specific regions of messenger RNA (mRNA) in a complex which also contains fMet-tRNA, GTP, initiation factors1 and involves the ribosomal proteins S1 and S12 (refs 2–5). Shine and Dalgarno proposed a mechanism for initiation of protein synthesis in which certain purine-rich nucleotide sequences near the initiator codon of prokaryotic messages were postulated to form base pairs with the nucleotide sequence (5′) ACCUCCUUA(3′) near the 3′ end of the 16S ribosomal RNA6,7. The formation, in vitro, of a complex between the 3′ end of the 16S rRNA from Escherichia coli ribosomes and the initiator region of phage R17 A-protein mRNA has been demonstrated8. This binding supports the involvement of such base pairing in the binding of mRNA to ribosomes, at least for this message.

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  1. The Rockefeller University, New York, New York, 10021

    JEFFREY V. RAVETCH & KAREN S. JAKES

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  1. JEFFREY V. RAVETCH
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  2. KAREN S. JAKES
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RAVETCH, J., JAKES, K. Intact 3′ end of 16S rRNA is not required for specific mRNA binding. Nature 262, 150–153 (1976). https://doi.org/10.1038/262150a0

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