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Fidelity of DNA replication catalysed in vitro on a natural DNA template by the T4 bacteriophage multi-enzyme complex

Nature volume 285, pages 300–305 (1980)Cite this article

Abstract

More than 50 copies of a ΦX174 DNA template can be made in 60 min in an in vitro DNA replication system consisting of seven purified replication proteins isolated from T4 bacteriophage-infected cells. By transfecting with the DNA products and assaying for the reversion of specific amber mutants, the high degree of base-pairing fidelity in this system is revealed; the in vitro system is also shown to respond to the mutagenic effect of Mn2+ and to display strong base-pair context effects on fidelity, as expected from in vivo studies.

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Author notes

  1. Urszula Hibner

    Present address: Parasitologie Expérimental, Institut Pasteur, 25 Rue du Dr Roux, 75015, Paris, France

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, 94143

    Urszula Hibner & Bruce M. Alberts

Authors
  1. Urszula Hibner
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  2. Bruce M. Alberts
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Hibner, U., Alberts, B. Fidelity of DNA replication catalysed in vitro on a natural DNA template by the T4 bacteriophage multi-enzyme complex. Nature 285, 300–305 (1980). https://doi.org/10.1038/285300a0

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