Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Protein-linked RNA of poliovirus is competent to form an initiation complex of translation in vitro

Nature volume 287, pages 600–603 (1980)Cite this article

Abstract

Poliovirus RNA that had been labelled with 125I in the 5′-terminal protein (VPg) was found competent to form an initiation complex of translation in a cell-free reticulocyte lysate. In conditions of ribosome binding, no cleavage occurred between VPg and RNA. We conclude that removal of VPg from poliovirus RNA is not a prerequisite for this RNA to initiate translation in vitro.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to the full article PDF.

USD 39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Jen, G., Birge, C. H. & Thach, R. E. J. Virol. 27, 640–647 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Lawrence, C. & Thach, R. E. J. Virol. 14, 598–610 (1974).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Golini, F. et al. Proc. natn. Acad. Sci. U.S.A. 73, 3040–3044 (1976).

    Article  ADS  CAS  Google Scholar 

  4. Lee, Y. F., Nomoto, A. & Wimmer, E. Prog. Nucleic Acid Res. molec. Biol. 19, 89–96 (1976).

    Article  CAS  Google Scholar 

  5. Lee, Y. F., Nomoto, A., Detjen, B. M. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 74, 59–63 (1977).

    Article  ADS  CAS  Google Scholar 

  6. Flanegan, J. B., Pettersson, R. F., Ambros, V., Hewlett, M. J. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 74, 961–965 (1977).

    Article  ADS  CAS  Google Scholar 

  7. Nomoto, A., Detjen, B., Pozzatti, R. & Wimmer, E. Nature 268, 208–213 (1977).

    Article  ADS  CAS  Google Scholar 

  8. Golini, F., Nomoto, A. & Wimmer, E. Virology 89, 112–118 (1978).

    Article  CAS  Google Scholar 

  9. Nomoto, A., Lee, Y. F. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 73, 375–380 (1976).

    Article  ADS  CAS  Google Scholar 

  10. Shih, D. S. et al. Proc. natn. Acad. Sci. U.S.A. 75, 5807–5811 (1978).

    Article  ADS  CAS  Google Scholar 

  11. Nomoto, A., Kitamura, N., Golini, F. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 74, 5345–5349 (1977).

    Article  ADS  CAS  Google Scholar 

  12. Pérez-Bercoff, R. & Gander, M. FEES Lett. 96, 306–312 (1978).

    Article  Google Scholar 

  13. Ambros, V., Pettersson, R. F. & Baltimore, D. Cell 15, 1439–1446 (1978).

    Article  CAS  Google Scholar 

  14. Weber, L. A., Simili, M. & Baglioni, C. in Meth. Enzym. 60, 351–360 (1979).

    Google Scholar 

  15. Stewart-Blair, M. L., Yanowitz, I. S. & Goldberg, I. H. Biochemistry 10, 4198–4206 (1971).

    Article  CAS  Google Scholar 

  16. Rothberg, P. G., Harris, T. J. R., Nomoto, A. & Wimmer, E. Proc. natn. Acad. Sci. U.S.A. 75, 4868–4872 (1978).

    Article  ADS  CAS  Google Scholar 

  17. Wimmer, E. in The Molecular Biology of Picornaviruses (ed. Pérez-Bercoff, R.) 175–190 (Plenum, New York, 1979).

    Book  Google Scholar 

  18. Pettersson, R. F., Ambros, V. & Baltimore, D. Virology 27, 357–366 (1978).

    CAS  Google Scholar 

  19. Baltimore, D. in Biochemistry of Viruses (ed. Levy, H. B.) 101–176 (Dekker, New York, 1969).

    Google Scholar 

  20. Pelham, H. R. B. & Jackson, R. J. Eur. J. Biochem. 67, 247–256 (1976).

    Article  CAS  Google Scholar 

  21. Darnbrough, C. H., Legon, S., Hunt, T. & Jackson, R. J. J. molec. Biol. 76, 379–403 (1973).

    Article  CAS  Google Scholar 

  22. Lee, Y. F., Kitamura, N., Nomoto, A. & Wimmer, E. J. gen. Virol. 44, 311–322 (1979).

    Article  CAS  Google Scholar 

  23. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  24. Bonner, W. M. & Laskey, R. A. Eur. J. Biochem. 46, 83–88 (1974).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Fred Golini

    Present address: Department of Cellular Biology, Scripps Clinic and Research Foundation, 10666 North Torrey Pines Road, La Jolla, California, 92037

Authors and Affiliations

  1. Department of Microbiology, State University of New York at Stony Brook, Stony Brook, New York, 11794

    Fred Golini, Bert L. Semler, Andrew J. Dorner & Eckard Wimmer

Authors
  1. Fred Golini
    View author publications

    Search author on:PubMed Google Scholar

  2. Bert L. Semler
    View author publications

    Search author on:PubMed Google Scholar

  3. Andrew J. Dorner
    View author publications

    Search author on:PubMed Google Scholar

  4. Eckard Wimmer
    View author publications

    Search author on:PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Golini, F., Semler, B., Dorner, A. et al. Protein-linked RNA of poliovirus is competent to form an initiation complex of translation in vitro. Nature 287, 600–603 (1980). https://doi.org/10.1038/287600a0

Download citation

  • Received:

  • Accepted:

  • Issue date:

  • DOI: https://doi.org/10.1038/287600a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing