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Regulation of metallothionein–thymidine kinase fusion plasmids injected into mouse eggs

Nature volume 296, pages 39–42 (1982) Cite this article

Abstract

A plasmid was constructed with the promoter/regulatory region of the mouse metallothionein-I gene fused to the structural gene of herpesvirus thymidine kinase. When mouse eggs were microinjected with this plasmid and incubated with cadmium (a natural inducer of metallothionein gene transcription) thymidine kinase activity increased ∼10-fold compared with control eggs not exposed to cadmium. Analysis of a set of deletion mutants revealed that the minimum sequence required for cadmium regulation lies within 90 nucleotides of the transcription start site.

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Authors and Affiliations

  1. Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA

    Ralph L. Brinster & Howard Y. Chen

  2. Howard Hughes Medical Institute Laboratory, Department of Biochemistry, University of Washington, Seattle, Washington, 98195, USA

    Raphael Warren, Aparna Sarthy & Richard D. Palmiter

Authors
  1. Ralph L. Brinster
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  2. Howard Y. Chen
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  3. Raphael Warren
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  4. Aparna Sarthy
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  5. Richard D. Palmiter
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Brinster, R., Chen, H., Warren, R. et al. Regulation of metallothionein–thymidine kinase fusion plasmids injected into mouse eggs. Nature 296, 39–42 (1982). https://doi.org/10.1038/296039a0

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