Abstract
Leprosy, a chronic infectious disease afflicting between 10 and 15 million people, is caused by the obligate intracellular parasite Mycobacterium leprae1. Although M. leprae was the first identified bacterial pathogen of man2, basic biochemical, immunological, diagnostic and therapeutic investigations have been severely limited because it remains one of the few human pathogens that have not been cultured in vitro. An M. leprae recombinant DNA expression library was constructed to provide a source of genes encoding proteins relevant for such studies. Monoclonal antibodies directed against M. leprae specific antigens3–7 have been used to isolate the genes encoding the five most immunogenic protein antigens of the leprosy bacillus. We report here that M. leprae specific epitopes recognized by all of 13 monoclonal antibodies tested were produced by recombinant phage in Escherichia coli.
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References
Bloom, B. R. & Godal, T. Rev. Infect. Dis. 5, 765–780 (1983).
Hansen, G. A. Norsk Mag. Laegezitensk. 4, 1–88, I-L III (1874).
Ivanyi, J., Morris, J. A. & Keen, M. in Monoclonal Antibodies Against Bacteria (eds Macario, A. J. L. & Macario, E. C.) (Academic, New York, 1984).
Gillis, T. P. & Buchanan, T. M. Infect. Immunity 37, 172 (1982).
Coates, A. R. M., Allen, B. W., Hewitt, J., Mitchison, D. A. & Ivanyi, J. Lancet ii, 167 (1981).
Young, D. B., Fohn, M. J., Khanolkar, S. R. & Buchanan, T. M. Clin. exp. Immun. (in the press).
Engers, H. et al. Infect. Immunity 48, 603–605 (1985).
Young, R. A. & Davis, R. W. Proc. natn. Acad. Sci. U.S.A. 80, 1194–1198 (1983).
Young, R. A. & Davis, R. W. Science 222, 778–782 (1983).
Young, R. A. & Davis, R. W. in Genetic Engineering: Principles and Techniques Vol. 7 (eds Setlow, J. & Hollaender, A.) 29–41 (Plenum, New York, 1985).
Young, R. A. et al. Proc. natn. Acad. Sci. U.S.A. 82, 2583–2587 (1985).
Draper, P. Int. J. Lepr. 44, 95–98 (1976).
Clark-Curtiss, J. E., Jacobs, W. R., Docherty, M. A., Ritchie, L. R. & Curtiss, R. J. Bact. 161, 1093–1102 (1985).
Huynh, T., Young, R. A. & Davis, R. W. in DNA Cloning Techniques: A Practical Approach (ed. Glover, D.) (IRL, Eynsham, in the press).
Bloom, B. R. & Mehra, V. in New Approaches to Vaccine Development (eds Bell, R. & Torrigiani, G.) 368–389 (Schwabe & Co., Basel, 1984).
Davis, R. W., Botsein, D. & Roth, J. R. Advanced Bacterial Genetics (Cold Spring Harbor Laboratory, New York, 1980).
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Young, R., Mehra, V., Sweetser, D. et al. Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae. Nature 316, 450–452 (1985). https://doi.org/10.1038/316450a0
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DOI: https://doi.org/10.1038/316450a0
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