Abstract
DNA replication in bacteriophage λ begins at a unique origin between residues 39,000 and 39,200 of the λ genome1. This segment of DNA serves a dual function since it also lies within the coding sequence of the λ replication initiator protein O which binds origin DNA2,3. The λ origin sequence contains four 19-base-pair (bp) segments (iterons) which have dyad symmetry, followed by a 40-bp A + T-rich zone of highly asymmetrical base composition2. It was noted earlier4 that λ origin DNA exhibits an anomalous electrophoretic mobility on gels; that is, the length of DNA as determined by DNA sequencing is ∼20% less than is predicted from electrophoretic mobility. Recent studies of kinetoplast minicircle DNA (K-DNA) from the protozoan Leishmania tarentolae have led to the proposal that sequence-induced DNA curvature could account for such electrophoretic anomalies by alteration of the shape of the DNA molecule5–8. We now present evidence that the λ origin contains a static curve.
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References
Schnös, M. & Inman, R. B. J. molec. Biol. 51, 61–73 (1970).
Moore, D. et al. Cold Spring Harb. Symp. quant. Biol. 43, 155–163 (1979).
Tsurimoto, T. & Matsubara, K. Nucleic Acids Res. 9, 1789–1799 (1981).
Daniels, D. L., deWet, J. R. & Blattner, F. R. J. Virol. 33, 39–400 (1980).
Marini, J. C., Levene, S. D., Crothers, D. M. & Englund, P. T. Proc. natn. Acad. Sci. U.S.A. 79, 7664–7668 (1982).
Marini, J. C. et al. J. biol. Chem. 259, 8974–8979 (1984).
Wu, H. & Crothers, D. M. Nature 308, 509–513 (1984).
Hagerman, P. J. Proc. natn. Acad. Sci. U.S.A. 81, 4632–4636 (1984).
Trifonov, E. N. & Sussman, J. L. Proc. natn. Acad. Sci. U.S.A. 77, 3816–3820 (1980).
Levene, S. D. & Crothers, D. M. J. biomolec. Struct. Dyn. 1, 429–435 (1983).
Denniston-Thompson, K., Moore, D. D., Kruger, K. E., Furth, M. E. & Blattner, F. R. Science 198, 1051–1056 (1977).
Gough, G. W. & Lilley, D. M. J. Nature 313, 154–156 (1985).
Grosschedl, R. & Hobom, G. Nature 277, 621–627 (1979).
Echols, H., Dodson, M., Better, M., Roberts, J. D. & McMacken, R. Cold Spring Harb. Symp. quant. Biol. 49, 727–733 (1984).
Trifinov, E. CRC Rev. Biochem. (in the press).
Sutcliffe, J. G. Cold Spring Harb. Symp. quant. Biol. 43, 77–90 (1979).
Norrander, J., Kempe, T. & Messing, J. Gene 26, 101–114 (1984).
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Zahn, K., Blattner, F. Sequence-induced DNA curvature at the bacteriophage λ origin of replication. Nature 317, 451–453 (1985). https://doi.org/10.1038/317451a0
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DOI: https://doi.org/10.1038/317451a0
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