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Cotransfection of heme oxygenase-1 prevents the acute inflammation elicited by a second adenovirus

Gene Therapy volume 10, pages 1629–1635 (2003)Cite this article

Abstract

The acute inflammatory response elicited by adenovirus vectors results in loss of gene expression and tissue injury in the target organ. This acute inflammation is now believed to be the major limiting factor for the use of adenovirus vectors in gene therapy. While exploring the level of acute inflammation caused by the adenovirus encoding the gene for the anti-inflammatory enzyme heme oxygenase-1, we discovered that this adenovirus not only did not elicit acute inflammation, but could prevent the inflammation caused by a second adenovirus. Here we describe a new approach to gene therapy, which uses the encoding of the potent anti-inflammatory enzyme heme oxygenase-1 to prevent early host inflammatory responses normally associated with adenovirus vectors.

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Acknowledgements

This work was supported by a grant from the Canadian Institutes of Health Research. Correspondence and requests for materials should be addressed to rpotter@uwo.ca.

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Authors and Affiliations

  1. Departments of Medical Biophysics and Surgery, University of Western Ontario and the Lawson Health Research Institute, London, ON, Canada

    S D McCarter, J R Scott, A Badhwar, A A Dungey, A Bihari, K A Harris & R F Potter

  2. Section of Pulmonary and Critical Care Medicine, Yale University School of Medicine, New Haven, CT, USA

    P J Lee & X Zhang

  3. Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    A M K Choi

  4. Department of Pathology, University of Western Ontario, London, ON, Canada

    C A McLean

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  1. S D McCarter
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  2. J R Scott
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McCarter, S., Scott, J., Lee, P. et al. Cotransfection of heme oxygenase-1 prevents the acute inflammation elicited by a second adenovirus. Gene Ther 10, 1629–1635 (2003). https://doi.org/10.1038/sj.gt.3302063

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