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Interaction between a transcriptional activator and transcription factor IIB in vivo

Nature volume 362, pages 549–553 (1993)Cite this article

Abstract

TRANSCRIPTION of messenger RNA-encoding genes in vitro requires many protein factors1,2. Transcription factor IID, possibly with the cooperation of TFIIA, binds to the TATA element of the promoter3–5, forming a complex that can bind TFIIB (refs 6,7) followed by RNA polymerase II (refs 6,8) and other factors9. One or more of these steps is thought to be facilitated by gene-specific transcriptional activation proteins10–12; this seems to require TFI ID-associated auxiliary factors13–15 and may involve direct contact between the activator and TFIID16,17 and/or TFIIB18,19. If such contact is necessary in vivo, activation might conceivably be blocked by a TFIIB derivative containing the sequences necessary for this interaction, but lacking those necessary for binding to the rest of the transcriptional apparatus, an effect similar to that referred to as squelching20 or transcriptional interference21. Here we show that the activity of the glutamine-rich fushi tarazu activation domain is indeed blocked by truncated TFIIB derivatives in Drosophila Schneider L2 cells, suggesting that it is mediated by interactions with TFIIB.

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Author notes

  1. Sharon Wampler: Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, California 92093, USA

Authors and Affiliations

  1. Department of Biological Sciences, Columbia University, New York, New York, 10027, USA

    John Colgan, Sharon Wampler & James L. Manley

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  1. John Colgan
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  2. Sharon Wampler
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  3. James L. Manley
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Colgan, J., Wampler, S. & Manley, J. Interaction between a transcriptional activator and transcription factor IIB in vivo. Nature 362, 549–553 (1993). https://doi.org/10.1038/362549a0

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