Figure 1
From: EGF-induced proliferation of adult human pancreatic duct cells is mediated by the MEK/ERK cascade
Expression of erb receptors in cultured adult human duct cells. (A) RT-PCR analysis of EGFR, ErbB2, ErbB3, ErbB4 expression in adult duct cells. RNA from duct cells was incubated for reverse transcription in the presence (RT+) or in the absence (RT−) of reverse transcriptase. Lane 1: 100 bp DNA ladder. The experimental conditions were described under Materials and methods. (B) Sections of human duct cell clusters cultured for 5 days in suspension and immunostained for EGFR (a) or ErbB3 (d). Staining of the duct cell marker CK19 is shown in consecutive sections (b, e). DAPI stains all nuclei blue. On control adjacent sections (c, f) primary antibodies were omitted. Bar, 25 μm.
