Figure 3
From: Expression of HSP47, a collagen-specific chaperone, in normal and diseased human liver
Immunoreactive HSP47 is detectable in control human liver and increased in abundance in cirrhosis. Detergent extracts of liver homogenates (20 μg protein) were separated in a 12% polyacrylamide gel and transblotted onto a nitrocellulose membrane. Immunoreactive HSP47 was detected using a monoclonal anti-HSP47 antibody as described in the Materials and methods. The signal in the first lane is the 43 kD molecular weight marker; the three lanes labeled ‘control’ are samples from normal donor livers; the remaining lanes are samples from cirrhotic livers removed at the time of liver transplant. ALD, alcoholic liver disease; HCV, chronic hepatitis C infection; PBC, primary biliary cirrhosis.
