Figure 5
From: Expression of HSP47, a collagen-specific chaperone, in normal and diseased human liver
HSP47 expression in activated human HSC is unaltered by profibrogenic agents and superoxide generators. Human HSC were cultivated as described in the Material and methods. After 24 h of the indicated treatments, HSC were lysed and HSP47 analyzed by Western blot as described in the Materials and methods (2 μg protein per well). Top panel, control, HSC cultured in serum-free medium; ANGII, HSC treated with angiotensin II (10 μM); TGFβ1, HSC treated with TGF-β1 (10 ng/ml). Bottom panel, control; HSC cultured in standard serum-containing medium; X/XO, HSC treated with xanthine (1 mM) and xanthine oxidase (1 mU/ml); Mena, HSC treated with menadione (5 μM); 1% O2, HSC cultured at 1% oxygen.
