Figure 6
From: Expression of HSP47, a collagen-specific chaperone, in normal and diseased human liver
Treatment of human HSC with antisense oligonucleotides to HSP47 lowers levels of HSP47 and is associated with a reduction in secreted α1(I) procollagen in the medium. Human HSC were transfected with sense and antisense oligonucleotides to HSP47 as described in the Materials and methods. HSC were harvested at various time points following transfection; in the experiment illustrated here, HSC and media were harvested 96 h after transfection. Cellular lysates were analyzed for HSP47 and β-actin and concentrated media for α1(I) procollagen by Western analysis as described in the Materials and methods.
