Figure 1
From: Matrix metalloproteinases, tissue inhibitors of MMPs and TACE in experimental cerebral malaria
Gelatin-zymography analysis of organ extracts of mice infected with P. berghei ANKA. C57Bl/6 mice were infected with P. berghei ANKA and killed at the indicated days after infection. Extracts of the organs of three mice at each time point were analysed by gelatin zymography to measure the amounts and the molecular forms of gelatinase A and B (MMP-2 and -9). The gelatinase B monomers (110?kDa) and dimers (220?kDa) are indicated with open arrowheads and activated monomers (95?kDa) with open arrowheads annotated with ‘act’. Gelatinase A is indicated with filled arrowheads. The molecular weights of a standard gelatinase preparation are indicated in kDa. Quantification by scanning densitometry of the zymolytic bands of progelatinase B (black bars), activated gelatinase B (hatched bars) and gelatinase A (grey bars) is shown in the graphs at the right, and the statistical differences with the uninfected controls are indicated (*P<0.05; **P<0.02; ***P<0.002). St, standard gelatinase preparation; Con, organ extracts of uninfected control mice; D2, 2 days after infection.
