Figure 2
From: Matrix metalloproteinases, tissue inhibitors of MMPs and TACE in experimental cerebral malaria
Gelatin-zymography analysis of brain extracts of mice infected with P. berghei ANKA and P. berghei NK65. (a) C57Bl/6 wild type or gelatinase B knockout or Balb/C wild-type mice were infected with P. berghei ANKA or with P. berghei NK65 and killed after 8 days. Extracts of the brains of these mice were analysed by gelatin zymography and compared with brain extracts of three uninfected control mice (Con) to measure the amounts and the molecular forms of gelatinase A and B (MMP-2 and -9), which are indicated. The faint and sharply delineated zymolytic band around 85?kDa in the brain extracts of the gelatinase B knockout mice is unrelated to gelatinase B. The molecular weights of a standard gelatinase preparation are indicated in kDa. St, standard gelatinase preparation. (b) The zymolytic bands shown in panel a were quantified by scanning densitometry. Significant differences with the uninfected controls and between ANKA- and NK65-infected C57Bl/6 mice and ANKA-infected Balb/c mice are indicated (*P<0.05; **P<0.02). Histogram colour code is as in Figure 1.
