Figure 3

Immunohistochemical localisation of gelatinase B in the brain of mice with CM. Frozen sections of mice with CM (a, c–f) or uninfected mice (b) were stained with an antiserum specific for gelatinase B (green). Sections of gelatinase B knockout mice (c) were used as negative controls. To further characterise the gelatinase B⁺ cells, double immunostaining with CD11b (red) was performed. In (d), the green fluorescence of gelatinase B⁺ cells in a double-stained section are shown, and in (e) the red fluorescence of CD11b⁺ cells is obvious in the same field. In (f), panels d and e were merged and colocalisation is indicated by the white arrows. (g) The number of gelatinase B⁺ cells were counted in 20 fields of cerebral cortex or cerebellum and compared between uninfected mice and mice with CM. ^*P<0.05; ^****P<2 × 10⁻⁶.