Figure 5
From: Matrix metalloproteinases, tissue inhibitors of MMPs and TACE in experimental cerebral malaria
Detection of organ-specific mRNA expression of MMPs and MT-MMPs during the development of CM in mice. C57Bl/6 mice were infected with P. berghei ANKA and spleen, lungs, kidneys and liver were dissected at the indicated times (three mice for each time point) and used for mRNA preparation. After electrophoresis, the mRNAs of different MMPs and MT-MMPs were detected using radiolabelled probes. The house-keeping gene RPL32 was used as a reference control. (a) A limited set of autoradiographs of some organs are shown as examples. (b) Autoradiographs for the expression of MMPs and MT-MMPs were scanned, quantified by densitometry (n=3 for each time point) and normalised with the RPL32 mRNA as reference. A selected set of averaged data, containing all observed alterations as a function of time, are shown. C, uninfected control; p.i., postinfection.
