Figure 6
From: Matrix metalloproteinases, tissue inhibitors of MMPs and TACE in experimental cerebral malaria
Detection of the mRNA of MMPs by RT-PCR in the brain during development of CM in mice. Brains from C57Bl/6 mice (three mice per indicated time point) infected with P. berghei ANKA were dissected and homogenised at the indicated time points during the course of the disease. mRNA was isolated, quantified and subjected to RT-PCR for soluble MMPs. Relative mRNA levels are indicated as fold increases compared to mRNA from uninfected mice. MMP-2 and -7 remained below the detection limit. 18S mRNA was used as a control for the quantification of total mRNA. *P<0.05; **P<0.02.
