Figure 8
From: Matrix metalloproteinases, tissue inhibitors of MMPs and TACE in experimental cerebral malaria
Alterations of the expression of TIMPs during the development of CM. After collection of organs of mice infected with P. berghei ANKA (three mice for each indicated time point), mRNA was isolated and used to document the expression of TIMP-1, -2, -3 and -4 and α2-macroglobulin by RPA and/or RT-PCR. (a) A limited set of autoradiographs of the RPA of some organs are shown. (b) Autoradiographs for the expression of TIMPs were scanned, quantified by densitometry and normalised with the RPL32 mRNA as reference. A selected set of averaged data, containing all observed alterations as a function of time, are shown. (c) RT-PCR analysis of the expression of TIMPs in the brain. 18S was used as a control for the mRNA concentration. C, uninfected control; p.i., post-infection; α2?M, α2-macroglobulin.
