Figure 9
From: Matrix metalloproteinases, tissue inhibitors of MMPs and TACE in experimental cerebral malaria
Localisation of gelatinolytic activity in the brain by in situ zymography. Frozen sections of C57Bl/6 mice with CM (8 days after infection) and of different control mice were incubated with quenched FITC-labelled gelatin. Local gelatinolytic activity resulted in dequenching and was visualised by fluorescence microscopy. (a) Cerebellum of wild-type mouse with CM; (b) higher magnification of panel a showing strongly staining blood vessels. (c) Cerebellum from a gelatinase B knockout mouse with CM. (d) Cerebellum from an uninfected control mouse. (e and f) Cortex (with a strongly staining blood vessel in the septum in e) from a wild-type mouse with CM; (g) Cortex from an uninfected control mouse. (h) In the presence of EDTA, no gelatinolytic activity was observed (the cerebellum from a wild-type mouse with CM is shown). (i) Cerebellum of uninfected Balb/C mouse and (j) cerebellum from a CM-resistant Balb/C mouse 8 days after infection with P. berghei ANKA.
