Figure 1 | Laboratory Investigation

Figure 1

From: Antibody validation by quantitative analysis of protein expression using expression of Met in breast cancer as a model

Figure 1

Met antibody validation on Western blot. (a) HEK293 cells express low levels of the Met receptor and its 60 kDa fragment. Antibodies 3D4, C12, CVD13 and C28 detect the Met receptor (145 kDa) and the 60 kDa fragment of Met in HEK293 cell lysate. MAB3729 detects the 60 kDa fragment but not the full length receptor under these conditions. 3D4 and C28 detect other bands that are known truncation forms of the Met receptor. The antibody DO24, directed at the extracellular domain of Met, does not detect Met on Western blots and is not shown. (b) Antibody epitopes to the Met receptor are depicted on the schematic representing the Met receptor.

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