Figure 3 | Laboratory Investigation

Figure 3

From: Antibody validation by quantitative analysis of protein expression using expression of Met in breast cancer as a model

Figure 3

Automated Quantitative Analysis (AQUA) of Met antibody MAB3729. (a) A representative tissue spot stained with cytokeratin is used to identify areas of tumor to create a tumor mask. (b) The tumor mask is compartmentalized into nuclear (blue) and non-nuclear (green) regions based on the location of DAPI staining within the tumor mask (c) Met expression (d) is placed within a compartment and the intensity of expression is given an AQUA score on a continuous scale of 0–255.

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