Table 1 Reproducibility of Met antibodies on Yale Tissue Microarray (YTMA) 49

From: Antibody validation by quantitative analysis of protein expression using expression of Met in breast cancer as a model

TMA pair

49_2 vs 49_3

49_2 vs 49_4

49_3 vs 49_4

49_3 vs 49_3

49_4 vs 49_4

49_3 vs 49_5

49_4 vs 49_5

(a)

       

DO-24

0.387

      

3D4

0.194

  

0.65

   

CVD13

0.294

0.168

0.58

    

C12

  

0.323

    

C28

      

0.29

MAB3729

  

0.48

 

0.8

0.55

0.57

(b)

       

TMA pair

49_2 vs 49_2

49_3 vs 49_3

49_4 vs 49_4

49_5 vs 49_5

   

DO-24 vs 3D4

0.51

0.671

     

DO-24 vs CVD13

0.293

0.076

     

DO-24 vs C12

 

0.225

     

3D4 vs CVD13

0.017

0.055

     

3D4 vs C12

 

0.314

     

CVD13 vs C12

 

0.145

0.32

    

MAB3729 vs 3D4

 

0.141

     

MAB3729 vs DO24

 

0.141

     

MAB3729 vs C12

 

0.115

0.36

    

MAB3729 vs CVD13

 

0.447

0.4

    

MAB3729 vs C28

  

0.71

0.62

   
  1. (a) Regression analysis of redundant histospots on different arrays or different sections of the same array. Only Met antibody MAB3729 had R values consistently above 0.5. (b) Regression analysis between Met antibodies on the same array. MAB3729 and C28 were the only Met antibodies showing correlation between expression levels.
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