Figure 1
From: Apoptosis Induced In Vitro and In Vivo During Infection by Ebola and Marburg Viruses
Analysis of lymph node sections from filovirus-infected monkeys by deoxyuridine triphosphate nick-end labeling (TUNEL) assay for apoptosis (A-D). Single-strand breaks appear blue/black. A, Follicle center of Ebola (EBO)-Z–infected monkey congested with tingible body macrophages (TBM). B, Follicle center of Marburg (MBG)-infected monkey showing few TBM and abundant free nuclear debris. C, Follicle center of EBO-Reston (R)–infected monkey with a few TBM and little free nuclear debris. D, Follicle center of uninfected control monkey. In E and F, labeling for apoptosis and immunohistochemistry (IHC) for filoviral antigen. E, Area from medulla of EBO-Zaire (Z)–infected monkey showing co-localization of EBO antigen (brown) and apoptotic cells. F, Follicle center of MBG-infected monkey showing co-localization of MBG antigen (brown) and apoptotic cells. In G and H, IHC for macrophage marker (shown in brown) using the Envision System (DAKO). Note increased numbers of macrophages in lymph node of EBO-Z–infected monkey (G) versus MBG-infected animal (H). Original magnifications, × 40 in A-F, × 20 in G and H.
