Figure 13

Examination of apoptosis using a wide-band green filter (A, C, E, and G) and double labeling for apoptosis and CD3 using a dual pass FITC/TRITC filter (B, D, F, and H) in mesenteric lymph nodes of filovirus-infected monkeys. Single-strand breaks are shown in red, CD3 appears green, and areas positive for both apoptosis and CD3 are stained gold. In A and B, section from uninfected control animal showed infrequent apoptotic cells (A) and no visible apoptotic CD3⁺ cells (B). The number of apoptotic cells was increased in EBO-Z– (C), EBO-R– (E), and MBG-infected (G) monkeys. Double labeling showed the presence of apoptotic CD3⁺ cells in follicles and perifollicular areas of EBO-Z– (D), EBO-R– (F), and MBG-infected (H) monkeys. Also, increased numbers of CD3⁺ cells are evident within the follicle centers of EBO-Z– (D) and EBO-R–infected (F) animals versus uninfected control monkey (B). Original magnifications, × 40 in A-H.