Figure 5
Methylation status at the AR locus on the X chromosome of PSTT by HUMARA analysis. (+) and (−) signs refer to +/- HhaI digestion. (A) Case #1: Lanes 1 and 2 are PCR products of tumor (T) and lanes 3 and 4 are products of control tissue (N). The paternal band (lower) is present only in tumor cells and is preferentially skewed upon HhaI digestion. (B) Case #4: The tumor cells (T) contribute an additional high molecular paternal band (upper band, lane 1) which does not exist in maternal normal control tissue (N, lanes 3 and 4). This paternal band seems preferentially skewed upon HhaI digestion (lane 2). (C) Case #2: The tumor harbors a unique paternal band (lane 1, lower band). The upper maternal band is preferentially hypomethylated while the lower paternal band is hypermethylated (lane 2, with HhaI digestion). The two normal maternal X chromosomes show no polymorphism at the AR locus (lane 3). The weak signal in normal tissue (lane 3 and 4) reflects severe degradation of the DNA in this case.
