Figure 5
From: Response of Nasopharyngeal Carcinoma Cells to Epstein-Barr Virus Infection In Vitro
RT-PCR analysis of growth factor and cytokine expression in NPC-TW 01 and NPC-TW 06 cell lines after EBV infection for 1, 2, 3, and 4 weeks, respectively. A, The gel analysis of RT-PCR product of EGFR-mRNA in NPC-TW 01 cell line. After EBV infection for 2–3 weeks, the EGFR mRNA of 677 band shows markedly increased intensity (lanes 3, 4). But 4 weeks after infection, the intensity of this band is decreased to the original level (lane 5). The internal control β-actin band has 318 bp. B, RT-PCR analysis of TGF-α mRNA expression in NPC-TW 06 cell line. After infection for 1 week, the TGF-α mRNA of 450 bp band is markedly elevated (lane 2), but gradually returns to the original level between 2–4 weeks after infection (lanes 3, 4, 5). The β-actin band of 318 bp is an internal control band. C, RT-PCR analysis of TGF-β1 mRNA expression in NPC-TW 06 cell line. The TGF-β1 mRNA expression of 288 bp band shows no specific change after EBV infections (lanes 2–5). The β-actin band of 173 bp is the internal control. D, RT-PCR analysis of IL-1β mRNA expression in NPC-TW 06 cell line. The intensity of 431 bp band of IL-1β mRNA is increased 1 week after EBV infection (lane 2), but gradually recovered to the original level 2, 3, and 4 weeks after infection (lanes 3, 4, 5). The 318 bp band of β-actin mRNA is an internal control. E, RT-PCR analysis of IL-6 mRNA expression in NPC-TW 06 cell line. The intensity of 495 bp band of IL-6 mRNA is increased after EBV infection for 1 week (lane 2). But it decreases gradually to the original level 2–4 weeks after infection (lanes 3, 4, and 5). The 318 bp band of β-actin mRNA is an internal control. F, RT-PCR analysis of granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA expression in NPC-TW 01 cell line. The intensity of 515 bp band of GM-CSF product is markedly increased 2–3 weeks after infection (lanes 2 and 3). It maintains the increased level, with slightly decreased intensity, 4 weeks after infection (lane 5). The 318 bp band of β-actin product is an internal control. Each experiment has been repeated 3 times.
