Table 1 Primers for Polymerase Chain Reaction
From: Response of Nasopharyngeal Carcinoma Cells to Epstein-Barr Virus Infection In Vitro
Primers | Sequence | Base | MgCl2 (mM) | Reference |
|---|---|---|---|---|
β-actin | 5′-ATCATGTTTGAGACCTTCAA-3′ | 318 bp | 1.5–2.0 | |
5′-CATCTCTTGCTCGAAGTCCA-3′ | ||||
β-actin | 5′-CGGGAAATCGTGCGTGACAT-3′ | 173 bp | 1.5–2.0 | |
5′-GAAGGAAGGCTGGAAGAGTG-3′ | ||||
EGF receptor | 5′-CTCATTGCCCTCAACACAGT-3′ | 677 bp | 1.5 | |
5′-CGTCTTCCTCCATCTCATAG-3′ | ||||
TGFα | 5′-TTCGCTCTGGGTATTGTGTT-3′ | 450 bp | 1.5 | |
5′-CTCCTCCTCTGGGCTCTTCA-3′ | ||||
TGF-β1 | 5′-CTCCGAGAAGCGGTACCTGAAC-3′ | 288 bp | 1.5 | |
5′-CACTTGCAGTGTGTTATCCCT-3′ | ||||
TGF-β receptor I | 5′-TGAGAAAAGTTGTTTGTGAAC-3′ | 160 bp | 1.5 | |
5′-TGACTGAGTTGCGATAATGT-3′ | ||||
TGF-β receptor II | 5′-AGGGACCTCAAGAGCTCCAATAT-3′ | 169 bp | 1.5 | |
5′-ATCCTGGATTCTAGGACTTCTGG-3′ | ||||
IL-1β | 5′-CGATCACTGAACTGCACGCTCCG-3′ | 431 bp | 1.5 | |
5′-GGTGAAGTCAGTTATATCCTGGCCG-3′ | ||||
IL-6 | 5′-CACACAGACAGCCACTCACCTC-3′ | 495 bp | 1.5 | |
5′-CTCAGGGTGGACTGCAGGAAC-3′ | ||||
IL-8 | 5′-GCAGCTCTGTGTGAAGGTGCAG-3′ | 365 bp | 1.5 | |
5′-GCATCTGGCAACCCTACAACAG-3′ | ||||
TNF-α | 5′-CTTCTGCCTGCTGCACTTTGGA-3′ | 547 bp | 1.5 | |
5′-TCCCAAAGTAGACCTGCCCAGA-3′ | ||||
GM-CSF | 5′-ATGTGAATGCCATCCAGGAGGC-3′ | 515 bp | 1.5 | |
5′-CCCATTCTTCTGCCATGCCTGT-3′ | ||||
EBV-Bam HI W | 5′-GTTCGCGTTGCTAGGCCACC-3′ | 110 bp | 2.5 | Lin et al, 1994 |
5′-AGGACCACTTTATACCAGGG-3′ |
