Table 1 Comparison of Fractions of Events in 5 Histogram Regionsa in 13 Paired U937 Samples of Whole Nonembedded Cells and Bare Cell Nuclei Extracted from Sections of Paraffin-Embedded Cells

From: Combined Analysis of DNA Ploidy, Proliferation, and Apoptosis in Paraffin-Embedded Cell Material by Flow Cytometry

 

Nonembedded whole cells*

Embedded cell nuclei*

Paired t-test P

% TUNEL-negative G1

36.9 ± 14.9

33.2 ± 17.5

0.042

% TUNEL-negative sub-G1

1.1 ± 0.6

4.6 ± 1.5

<0.001

% TUNEL-positive G1

10.5 ± 8.6

3.1 ± 3.6

0.002

% TUNEL-positive sub-G1

13.6 ± 13.2

29.2 ± 22.3

0.004

% all TUNEL-positive events

31.4 ± 24.6

37.1 ± 28.1

0.041

  1. aThe histogram regions of TUNEL-positive and -negative G1 and sub-G1 events are indicated in Figure 4 with broken lines; the histogram region of all TUNEL-positive events corresponds to the range above the solid line. S- and G2-phase events were not evaluated separately. G1, G1-phase fraction.
  2. ** Mean values ± sd.
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