Figure 2 | Laboratory Investigation

Figure 2

From: Expression of Presumed Specific Early and Late Factors Associated with Liver Regeneration in Different Rat Surgical Models

Figure 2

A, Identification of signal transducer and activator of transcription 3 (STAT3) binding activity by EMSA. Ten micrograms of nuclear proteins from control (Lane 1), SO (0.5 and 2 hours, Lanes 2 and 3), and PCS (0.5 and 2 hours, Lanes 4 and 5) were incubated with a 32P-labeled oligonucleotide containing the STAT3 binding site followed by electrophoresis through a 5% polyacrylamide gel. Supershift experiments with STAT3- and STAT5-specific antibody confirmed that the complexes detected after SO (Lanes 6–8) and PCS did contain STAT3, but not STAT5. B, Quantification of STAT3 protein by densitometric analysis of Western blots. Mean values ± sem obtained from individual densitometric measures are represented (n = 3). Weak expression of this factor was noticed in control animals. STAT3 protein was significantly induced 2 hours after PH, SO, and PCS. *p < 0.05; **p < 0.01; ***p < 0.001.

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