Figure 3

Expression of interleukin-6 (IL-6) mRNA. Representation of reverse transcription-polymerase chain reaction (RT-PCR) of IL-6 and ribosomal protein L19 (RPL-19) mRNAs in controls (Lane 1), 0.5 hours and 2 hours after SO (Lanes 2 and 3), and 0.5 hours and 2 hours after PCS (Lanes 4 and 5). Total liver mRNA was subjected to RT-PCR as described in “Materials and Methods.” The results were adjusted for their respective RPL-19 levels and then compared to a standard dilution curve obtained by amplification of liver mRNA from lipopolysaccharide (LPS)-treated rats in the same PCR procedure. IL-6 expression was significantly increased at 2 hours after SO and PCS (p < 0.05).