Figure 1 | Laboratory Investigation

Figure 1

From: Glial Cell Line–Derived Neurotrophic Factor Family Receptors Are Abnormally Expressed in Aganglionic Bowel of a Subpopulation of Patients with Hirschsprung's Disease

Figure 1

In situ hybridization analysis of GFRA1 expression in normal infant colon. Adjacent sections were immunostained with anti-S100 antibody (A and C) or hybridized with GFRA1 riboprobe (B and D). Glial cells in the ganglion plexuses are smaller with elongated nuclei (black arrowheads) and were S100 immunopositive. Neuronal cells are bigger with round nuclei (white arrowheads) and were not immunostained with anti-S100 antibody. Transcripts of GFRA1 were localized to glial (black arrowheads) and neuronal cells (white arrowheads) of myenteric plexus (B) and submucosal plexus (D). Sense riboprobe of GFRA1 showed no specific hybridization (E). Photographs of the hybridized sections were taken under bright field illumination. Scale bars represent 10 μm.

Back to article page