Figure 3 | Laboratory Investigation

Figure 3

From: Raft.1, A Monoclonal Antibody Raised Against the Raft Microdomain, Recognizes G-Protein β1 and 2, Which Assemble Near Nucleus After Shiga Toxin Binding to Human Renal Cell Line

Figure 3

Antigen analysis of 2-D Western blotting. a, Five micrograms of raft proteins were separated by IEF in the first dimension and then by SDS PAGE in the second dimension. The gel was fixed in 50% methanol/10% acetic acid/water and then silver-stained. b, The proteins (5 μg) separated as above were transferred to a nitrocellulose membrane and probed with Raft.1. c, Approximately 100 μg of raft proteins were separated by 2-D PAGE in the same manner and stained with CBB. Three circles indicate the spots to be subjected to MALDI-TOF MS analysis. The circle indicated by the arrowhead corresponds with the Raft.1 antigen. A indicates actin molecules used as a positive control for the analysis. Blank indicates the gel used for identification of the background peaks.

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