Figure 7 | Laboratory Investigation

Figure 7

From: Raft.1, A Monoclonal Antibody Raised Against the Raft Microdomain, Recognizes G-Protein β1 and 2, Which Assemble Near Nucleus After Shiga Toxin Binding to Human Renal Cell Line

Figure 7

Subcellular distribution of Gβ1 and 2 by sucrose density gradient centrifugation analysis. a, Triton lysates from whole cells (i), plasma membranes (ii), and the other microsome fractions (iii) were separated by sucrose density gradient centrifugation. Each fraction was analyzed by Western blotting and probed with T-20. b, Raft proteins prepared from plasma membrane (DIM in ii) were separated by 2-D PAGE and were silver stained.

Back to article page