Figure 9 | Laboratory Investigation

Figure 9

From: Raft.1, A Monoclonal Antibody Raised Against the Raft Microdomain, Recognizes G-Protein β1 and 2, Which Assemble Near Nucleus After Shiga Toxin Binding to Human Renal Cell Line

Figure 9

Raft.1 immunostaining in various rat organs (a) and comparison of the immunoreactivity and specificity of Raft.1 with G4 (b). a, The frozen sections of retina (i), testis (ii), heart (iii), liver (iv), hair follicle (v), and kidney (vi) obtained from a male rat were fixed in cold acetone and immunostained with Raft.1. (i) was counterstained with methyl green and the others with hematoxylin. The insets in (ii), (iii), (iv), and (vi) show positive immunostaining of the middle piece of sperm, cross striations of heart muscle, adjacent hepatic cells, and renal tubules, respectively. glc, ganglion cells; ipl, inner plexiform layer; opl, outer plexiform layer; olm, outer limiting membrane; rcs, rod and cone segments; mp, middle piece; hs, hair sheath; hp, hair papilla; g, glomerulus; rt, renal tubule. b, Frozen sections of rat brain were immunostained with Raft.1 (left) or G4 (right) and counterstained with hematoxylin. The inset in the right panel shows the positive staining on the apical side of the choroid plexus. cp, choroid plexus; ep, ependymal.

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