Figure 6 | Laboratory Investigation

Figure 6

From: Recombinant Adenovirus Vector Bearing Antisense Macrophage Migration Inhibitory Factor cDNA Prevents Acute Lipopolysaccharide-Induced Liver Failure in Mice

Figure 6

Effect of AxCA-mMIFS and AxCA-mMIFAS on the survival rate in mice primed with BCG and challenged with LPS. Each group of 30 mice received an injection of 2.0 × 109 pfu of AxCA-mMIFS, AxCA-mMIFAS, AxCA-LacZ, or PBS (Ca2+[−], Mg2+ [−])−10% glycerol buffer in addition to injection of BCG before LPS challenge to examine the effect of AxCA-mMIFS and AxCA-mMIFAS on BCG-LPS–induced acute liver failure. The survival rate at 48 hours after 10 μg of LPS injection after 7 days of priming with BCG was significantly high in mice infected with AxCA-mMIFAS (- - -) compared with the survival rate in mice infected with AxCA-mMIFS (—). The mice infected with AxCA-mMIFAS were more resistant to LPS challenge than mice treated with PBS (-----) and infected with AxCA-LacZ (– · – · –). The mice treated with AxCA-mMIFS were more lethal to LPS challenge than mice with PBS and AxCA-LacZ. p < 0.001 for difference between AxCA-mMIFAS (n =30) and AxCA-LacZ (n =30), AxCA-mMIFAS (n =30) and AxCA-mMIFS (n =30). p < 0.01 for difference between AxCA-mMIFS (n =30) and AxCA-LacZ (n =30).

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