Figure 2 | Laboratory Investigation

Figure 2

From: High Frequency of TGF-β-Receptor-II Mutations in Microdissected Tissue Samples from Laryngeal Squamous Cell Carcinomas

Figure 2

Results of the single-stranded conformation polymorphism (SSCP) analysis. (A) In the first set of experiments, artificial mutations were induced during the amplification process. Although a normal banding pattern is seen in the controls using normal primers (Lanes 1 to 4), the exclusive application of primers introducing mutation (Lane 5) results in only shifted bands (arrows). The application of both mutated and normal primers shows both normal and abnormal bands (Lane 6, exon 5). (B) SSCP gel showing the amplification products of a series of samples for exon 5. Here, no abnormal banding pattern is seen. (C) In a further set of samples, the amplification product of exon 5 reveals an abnormal banding pattern in two cases (Lanes 6 and 10), with the typical occurrence of additional bands (arrows). (D) One sample (Lane 2) shows exclusively two abnormally running bands (arrows). Note that in this gel the two regular SSCP bands are running close together.

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