Table 2 Primer Pairs for Molecular Analysisa

From: High Frequency of TGF-β-Receptor-II Mutations in Microdissected Tissue Samples from Laryngeal Squamous Cell Carcinomas

Exon

Upper primer

Lower primer

Annealing temperature (°C)

Product size (bp)

Exon size (bp)

1

5′-CAT CTG GCC CGC ACA TCT-3′

5′-CGG GCT GGG GAC CAC TC-3′

62

248

94

2

5′-CTG GCA GTT GGA TAA TCA TT-3′

5′-GGG GAA AAG AAA GAA TAA CTT-3′

60

252

169

3

5′-CCC TCG CTT CCA ATG AAT CTC-3′

5′-CCC ACA CCC TTT AGA GAA GAA-3′

60

250

191

4Ab

5′-CAA CTC CTT CTC TCC TTG TT-3′

5′-GCA GCT CTG TGT TGT GG-3′

55

305

 

4Bb

5′-TCC ACG TGT GCC AAC-3′

5′-TGC CGC GTC AGG TA-3′

58

335

800

4Cb

5′-CCT TCC ACG CCA AG-3′

5′-TCA AGG TAA AGG GGA TCT-3′

58

315

 

5Ac

5′-AGG GGC CAC CAT CAG-3′

5′-TAA CCC CTG GAA TAA TGC TC-3′

64

311

 

5Bc

5′-GGC CTC ACT GTC TGT TTT TG-3′

5′-TAC ACA TAT CTG GTC CAC ACC T-3′

64

192

142

6

5′-ACA TGC CAT TCT CAG TGA C-3′

5′-GAA TTC TCT GCC ACC TAA GA-3′

55

241

128

7

5′-GCG GAC CCA CCA ACT CAT-3′

5′-CCA GCC TGC CCC ATA AGA-3′

55

248

180

  1. GenBank accession numbers: U-52240 to U-52246 and U-69146 to U-69152.
  2. bExon 4 was divided into three amplification segments with overlapping sequences.
  3. cFor exon 5 a nested PCR was performed; A indicates the outer and B the inner primer pairs.