Table 1 Antibodies used in the present study
Antibody | Clone | Supplier | Dilution | Antigen retrieval |
|---|---|---|---|---|
αSMA | 1A4 | DAKO | 1:50 | Microwave |
p63 | 4A4 | Pharmingen | 1:200 | Microwave |
Maspin | G167-70 | Pharmingen | 1:150 | Microwave |
CK8 | TS1 | Novocastra | 1:100 | Microwave |
CK7 | OV-TL20/30 | DAKO | 1:25 | Trypsin |
CK19 | b170 | Novocastra | 1:100 | Trypsin |
CK14 | LL002 | Novocastra | 1:20 | Microwave |
CD44v6 | F10 44-2 | Novocastra | 1:100 | Microwave |
CD10 | NCL-CD10 | Novocastra | 1:40 | Microwave in EDTA buffer |
Calponin | CALP | Novocastra | 1:50 | Trypsin |
Caldesmon | TD107 | Novocastra | 1:50 | Microwave |
14-3-3σ | Polyclonal (N-14) | Santa Cruz | 1:150 | Microwave |
