Table 1 Antibodies used in this study
Marker | Source | Type | Dilution | Antigen retrieval |
|---|---|---|---|---|
D2-40 | Signet Laboratories (Dedham, MA, USA) | MAb | 1:25 | Yes (Tris-EDTA) |
Podoplanin | AngioBio Company (Del Mar, CA, USA) | MAb | 1:40 | Yes (citrate) |
Calretinin | Zymed (South San Francisco, CA, USA) | PAb (rabbit) | 1:20 | Yes (citrate) |
Keratin 5/6 | Dako Corporation (Carpinteria, CA, USA) | D5/16B4 MAb | 1:25 | Yes (citrate) |
Thrombomodulin | Dako Corporation | 1005 MAb | 1:50 | Yes (citrate) |
BG-8 (LewisY) | Signet Laboratories | BG-8 MAb | 1:50 | Yes (citrate) |
MOC-31 | Dako Corporation | MAb | 1:50 | Yes (citrate) |
CA19-9 | Dako Corporation | MAb | 1:50 | No |
TAG-72 | BioGenex (San Ramon, CA, USA) | B72.3 MAb | 1:300 | No |
CD15 | Becton-Dickinson (Mountainview, CA, USA) | Leu-M1 MAb | 1:40 | Yes (Tris-EDTA) |
Ber-EP4 | Dako Corporation | MAb | 1:30 | Yes (enzymatic digestion) |
