Table 1 PCR primers used in this study
Gene | Primer sequences | Annealing temp (°C) | Product size (bp) |
|---|---|---|---|
p27 | F: 5′-AGGACACGCATTTGGTGGA-3′ | 61 | 108 |
R: 5′-TAGAAGAATCGTCGGTTGCAGGT-3′ | |||
skp2 | F: 5′-GAAACGGCTGAAGAGCAAAG-3′ | 60 | 101 |
R: 5′-GAAGGGAGTCCCATGAAACA-3′ | |||
c-myc | F: 5′-TCAAGAGGCGAACACACAAC-3′ | 60 | 110 |
R: 5′-GGCCTTTTCATTGTTTTCCA-3′ | |||
18SrRNA | F: 5′-GGACACGGACAGGATTGACA-3′ | 60 | 50 |
R: 5′-ACCCACGGAATCGAGAAAGA-3′ | |||
IL-8 | F: 5′-GCCAACACAGAAATTATTGTAAAGCTT-3′ | 54 | 112 |
R: 5′-AATTCTCAGCCCTCTTCAAAAACTT-3′ |
